Figure 8.

UB organoids generated from iPSCs derived from a patient with ADPKD exhibit cystogenesis upon forskolin treatment. (A) Flow cytometry analysis of UB organoids generated from iPSCs derived from a patient with ADPKD (patient 4) at day 6.25. Wild-type human iPSCs (201B7) were used as controls. The percentages of the CXCR4⁺/KIT⁺ Wolffian duct progenitor fraction were determined from three independent induction experiments (mean±SEM). (B) Representative bright-field images of UB organoids upon forskolin treatment from day 18.5 to day 30. Scale bars, 200 μm. (C) Frequency of cyst formation upon forskolin treatment (n=32 in four independent experiments). Data are shown as mean±SEM. **P<0.01. (D and E) Confocal images of immunofluorescence in UB organoids at day 30 with staining for (D) GATA3 (green) and PAX2 (red) or (E) RET (green) and KRT19 (red). The asterisks indicate the lumens of cysts. Scale bars, 50 μm.