Figure 6.

CS/HA NP delivery of BMP7 or HGF/NK1 gene therapy attenuates renal fibrosis. (A) Immunohistology to visualize PCNA (top panel), α-SMA (middle panel), and fibronectin (bottom panel) in kidney cortex sections 7 days post UUO surgery (n=5). Scale bars, 100 µm. The percentage of kidney cortex positive for (B) PCNA, (C) α-SMA, and (D) fibronectin expression was quantified from three separate fields of view (n=5). (E) A panel of fibrosis-associated genes was assessed by qRT-PCR: Acta2, Fn1, Eda-Fn, Tgfb1 (n=5). Data are shown as mean±SD, and statistical significance is indicated by: *P≤0.05; **P≤0.01; ***P≤0.001.