Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Phospho-PKA Substrate (RRXS*/T*) (100G7E) rabbit monoclonal |
Cell Signaling Technology | RRID:AB_331817 | WB (1:1000) |
| Antibody | AMPA Receptor 1 (GluA1) (D4N9V) rabbit monoclonal | Cell Signaling Technology | RRID:AB_2732897 | WB (1:1000) |
| Antibody | Phospho-AMPA Receptor 1 (GluA1) (Ser845) (D10G5) rabbit monoclonal |
Cell Signaling Technology | RRID:AB_10860773 | WB (1:1000) |
| Antibody | Synaptophysin rabbit polyclonal | Cell Signaling Technology | RRID:AB_1904154 | WB (1:1000) |
| Antibody | Anti-RAB8A antibody [EPR14873] rabbit monoclonal | Abcam | RRID:AB_2814989 | WB (1:1000) |
| Antibody | RAB8A (phospho T72) [MJF-R20] rabbit monoclonal | Abcam | RRID:AB_2814988 | WB (1:1000) |
| Antibody | Rab10 (D36C4) XP Rabbit mAb rabbit monoclonal | Cell signaling technology | RRID:AB_10828219 | WB (1:1000) |
| Antibody | Anti-RAB10 (phospho T73) antibody [MJF-R21] rabbit monoclonal | Abcam | RRID:AB_2811274 | WB (1:1000) |
| Antibody | HOMER1 polyclonal antibody rabbit polyclonal | Proteintech | RRID:AB_2295573 | WB (1:1000) |
| Antibody | GluA1/GluR1 glutamate receptor clone N355/1 mouse monoclonal |
UC Davis/NIH NeuroMab Facility | RRID:AB_2315840 | IF (1:300) |
| Antibody | PSD-95 monoclonal (6G6-1C9) mouse monoclonal | Invitrogen | RRID:AB_325399 | WB (1:1000) IF (1:300) |
| Antibody | PSD-95 polyclonal rabbit polyclonal | Invitrogen | RRID:AB_87705 | IF (1:300) |
| Antibody | GFP chicken polyclonal | Invitrogen | RRID:AB_2534023 | IF (1:1000) |
| Antibody | mCherry (16D7) rat monoclonal | Invitrogen | RRID:AB_2536611 | IF (1:1000) |
| Antibody | Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP | Invitrogen | RRID:AB_2533947 | WB (1:5000) |
| Antibody | Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP | Invitrogen | RRID:AB_2533967 | WB (1:5000) |
| Antibody | Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 | Invitrogen | RRID:AB_162542 | IF (1:300) |
| Antibody | Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 | Invitrogen | RRID:AB_2534017 | IF (1:300) |
| Antibody | Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 | Invitrogen | RRID:AB_2534121 | IF (1:300) |
| Antibody | Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 488 | Invitrogen | RRID:AB_2534096 | IF (1:300) |
| Antibody | Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Invitrogen | RRID:AB_2535792 | IF (1:300) |
| Other | Fetal Bovine Serum | Sigma-Aldrich | F0926 | 5% |
| Other | Horse Serum, heat inactivated, New Zealand origin | Gibco | 26050088 | 5% |
| Peptide, recombinant protein | Laminin Mouse Protein, Natural | Gibco | 23017015 | 10 μg/ml |
| Chemical compound, drug | Poly-D-lysine hydrobromide | Sigma | P0899 | 50 μg/ml |
| Chemical compound, drug | GlutaMAX Supplement (100x) | Gibco | 35050061 | |
| Chemical compound, drug | B-27 Supplement (50X), serum free | Gibco | 17504044 | |
| Chemical compound, drug | N-2 Supplement (100X) | Gibco | 17502001 | |
| Chemical compound, drug | Antibiotic Antimycotic Solution (100×), Stabilized | Sigma-Aldrich | A5955 | |
| Chemical compound, drug | Basal Medium Eagle | Sigma-Aldrich | B1522 | |
| Chemical compound, drug | Cytosine β-D-arabinofuranoside | Sigma-Aldrich | C1768 | 2.5 μM |
| Chemical compound, drug | PDS Kit, Papain Vial | Worthington Biochemical Corporation | LK003178 | |
| Chemical compound, drug | DNase Vial (D2) | Worthington Biochemical Corporation | LK003172 | |
| Chemical compound, drug | Sucrose | Sigma-Aldrich | S7903 | |
| Chemical compound, drug | HEPES solution | Sigma-Aldrich | H0887 | |
| Chemical compound, drug | UltraPure 1M Tris-HCI, pH 8.0 | Invitrogen | 15568025 | |
| Chemical compound, drug | MgCl2 | Sigma-Aldrich | M8266 | |
| Chemical compound, drug | Calcium chloride solution | Sigma-Aldrich | 21115 | |
| Chemical compound, drug | Triton X-100 | Sigma-Aldrich | X100 | |
| Chemical compound, drug | Ethylenediaminetetraacetic acid disodium salt solution | Sigma-Aldrich | E7889 | |
| Chemical compound, drug | Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) | Thermo Scientific | 78441 | |
| Chemical compound, drug | Tris Buffered Saline (10x) | Sigma-Aldrich | T5912 | |
| Chemical compound, drug | TWEEN 20 | Sigma-Aldrich | P1379 | |
| Chemical compound, drug | MNI-L-glutamate | Tocris | 1490 | |
| Chemical compound, drug | Alexa Fluor 594 | Thermo Fisher Scientific | A10438 | |
| Chemical compound, drug | Sodium chloride | Sigma-Aldrich | S3014 | |
| Chemical compound, drug | Potassium chloride | Sigma-Aldrich | P9541 | |
| Chemical compound, drug | Sodium bicarbonate | Sigma-Aldrich | S5761 | |
| Chemical compound, drug | Sodium phosphate monobasic | Sigma-Aldrich | S3139 | |
| Chemical compound, drug | Calcium chloride | Sigma-Aldrich | C5670 | |
| Chemical compound, drug | D-(+)-Glucose | Sigma-Aldrich | G7021 | |
| Chemical compound, drug | Cesium methanesulfonate | Sigma-Aldrich | C1426 | |
| Chemical compound, drug | Cesium chloride | Sigma-Aldrich | C3032 | |
| Chemical compound, drug | HEPES | Sigma-Aldrich | 54457 | |
| Chemical compound, drug | Ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid | Sigma-Aldrich | E3889 | |
| Chemical compound, drug | Gabazine/SR 95531 hydrobromide | Tocris | 1262 | |
| Chemical compound, drug | Scopolamine hydrobromide | Tocris | 1414 | |
| Chemical compound, drug | Phosphocreatine disodium salt hydrate | Sigma-Aldrich | P7936 | |
| Chemical compound, drug | QX-314 Chloride | Tocris | 2313 | |
| Chemical compound, drug | Adenosine 5’-triphosphate magnesium salt | Sigma-Aldrich | A9187 | |
| Chemical compound, drug | Guanosine 5’-triphosphate sodium salt hydrate | Sigma-Aldrich | 51120 | |
| Chemical compound, drug | (R)-CPP | Tocris | 0247 | |
| Chemical compound, drug | Tetrodotoxin | Tocris | 1078 | |
| Chemical compound, drug | Potassium gluconate | Sigma-Aldrich | 1550001 | |
| Commercial assay or kit | iBlot Transfer Stack, nitrocellulose, regular size | Invitrogen | IB301031 | |
| Commercial assay or kit | NuPAGE 4–12% Bis-Tris Protein Gels, 1.5 mm, 15-well | Invitrogen | NP0336BOX | |
| Commercial assay or kit | NuPAGE MES SDS Running Buffer (20X) | Invitrogen | NP0002 | |
| Commercial assay or kit | NuPAGE Antioxidant | Invitrogen | NP0005 | |
| Commercial assay or kit | NuPAGE Transfer Buffer (20X) | Invitrogen | NP0006 | |
| Commercial assay or kit | NuPAGE LDS Sample Buffer (4X) | Invitrogen | NP0008 | |
| Commercial assay or kit | NuPAGE Sample Reducing Agent (10X) | Invitrogen | NP0009 | |
| Commercial assay or kit | Pierce BCA Protein Assay Kit | Thermo Scientific | 23225 | |
| Commercial assay or kit | Restore Western Blot Stripping Buffer | Thermo Scientific | 21059 | |
| Other | ProLong Diamond Antifade Mountant with DAPI | Invitrogen | P36971 | |
| Other | BLUeye Prestained Protein Ladder | Sigma-Aldrich | 94964 | |
| Commercial assay or kit | Immobilon ECL Ultra Western HRP Substrate | Millipore | WBULS0500 | |
| Software, algorithm | GraphPad Prism 7 | GraphPad Software Inc | RRID:SCR_002798 | |
| Software, algorithm | NIS-elements 5.10 | Nikon Instruments Inc | RRID:SCR_014329 | |
| Software, algorithm | Imaris 9.21 | Bitplane Inc | RRID:SCR_007370 | |
| Software, algorithm | iBright Analysis Software | Thermo Scientific | RRID:SCR_017632 | |
| Software, algorithm | MATLAB | MathWorks | RRID:SCR_001622 | |
| Software, algorithm | FIJI | Schindelin et al., 2012 | http://fiji.sc/; RRID:SCR_002285 | |
| Strain, strain background | rAAV8/Flex-GFP | UNC GTC vector core | Lot, AV4910B |