Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (S. pombe) | 972 | Laboratory stock | ATCC24843 | Stocked in T. Kanki lab. |
| Strain, strain background (S. pombe) | CA101 | Laboratory stock |
h- leu1-32
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | CA103 | Laboratory stock |
h- ura4-D18
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | MM72-1D | NBPR (https://yeast.nig.ac.jp/yeast/) | FY7652 |
h- leu1-32 ura4-D18
Stocked in T. Kanki lab. |
| Strain, strain background (S. pombe) | TFSP407 | This study |
h- ura4-D18 tuf1-mRFP::ura4
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1079 | This study |
h- ura4-D18 tuf1-mRFP::ura4 atg43-1::kanMX4
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1669 | This study |
h- ura4-D18 tuf1-mRFP::ura4 tom70Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP669 | This study |
h- ura4-D18 tuf1-mRFP::ura4 sdh2-mEGFP::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3834 | This study |
h- ura4-D18 tuf1-mRFP::ura4 sdh2-mEGFP::nat atg43-1::kanMX4
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1013 | This study |
h- atg43-1::kanMX4
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1057 | This study |
h- ura4-D18 tuf1-mRFP::ura4 atg43Δ::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3353 | This study |
h- ura4-D18 tuf1-mRFP::ura4 atg43-1::kanMX4 pNATZA31
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1941 | This study |
h- ura4-D18 tuf1-mRFP::ura4 atg43-1::kanMX4 pNATZA31-atg43
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3473 | This study |
h- atg43Δ::kan pNATZA31
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP2705 | This study |
h- atg43Δ::kan pNATZA31-atg43
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP183 | This study |
h- ura4-D18 GFP-atg8::ura4
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP991 | This study |
h- ura4-D18 GFP-atg8::ura4 atg43-1::kanMX4
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP743 | This study |
h- pgk1-mEGFP::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP989 | This study |
h- pgk1-mEGFP::hph atg43-1::kanMX4
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP671 | This study |
h- ura4-D18 tuf1-mRFP::ura4 yop1-mEGFP::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP987 | This study |
h- ura4-D18 tuf1-mRFP::ura4 yop1-mEGFP::nat atg43-1::kanMX4
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP25 | This study |
h- sdh2-mEFGP::kan tuf1-mRFP::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP137 | This study |
h- sdh2-mEFGP::kan tuf1-mRFP::hph atg1Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP145 | This study |
h- sdh2-mEFGP::kan tuf1-mRFP::hph isp6Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1273 | This study |
h- tom70-mEGFP::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | CA1258 | This study |
h- tuf1-mRFP::kan
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP78 | This study |
h- tuf1-mRFP::kan fun14Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1321 | This study |
h- tom70-mEGFP::hph atg43-1::kanMX4
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3475 | This study |
h- mic60-mEGFP::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3477 | This study |
h- mic60-mEGFP::nat atg43-1::kanMX4
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP209 | This study |
h- atg1Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP271 | This study |
h- isp6Δ::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP667 | This study |
h- ura4-D18 tdh1-mRFP::ura4 sdh2-mEGFP::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP985 | This study |
h- ura4-D18 tdh1-mRFP::ura4 sdh2-mEGFP::nat atg43-1::kanMX4
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1677 | This study |
h- ura4-D18 GFP-atg8::ura4 tom70Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1675 | This study |
h- pgk1-mEGFP::kan tom70Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1679 | This study |
h- ura4-D18 tuf1-mRFP::ura4 yop1-mEGFP::nat tom70Δ::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | CA9659 | DOI:10.1111/j.1365–2443.2006.01025.x | h+ ura4-D18 tor2-13::ura4 | |
| Strain, strain background (S. pombe) | TFSP1539 | This study |
h- atg7Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1071 | This study |
h- ura4-D18 tuf1-mRFP::ura4 hph::P81nmt:GFP-atg43
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3237 | This study |
h- ura4-D18 tuf1-mRFP::ura4 hph::P81nmt:GFP-atg43 atg7Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3177 | This study |
h- tom70-mEGFP::hph mic60-FLAG::kan tuf1-mRFP::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1097 | This study |
h- hph::P3nmt1:FLAG-atg43
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1717 | This study |
h- kan::P3nmt1:atg43-FLAG::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP2633 | This study |
h- hph::P3nmt1:FLAG-atg43(C80)
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3593 | This study |
h- kan::P3nmt1:atg43(C80)-FLAG::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | CA12620 | This study |
h+ ura4-D18 tor2-13::ura4 tuf1-mRFP::kan
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1399 | This study |
h- ura4-D18 tuf1-mRFP::ura4 hph::P3nmt1:atg43
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1101 | This study |
h- ura4-D18 tuf1-mRFP::ura4 hph::P3nmt1:FLAG-atg43
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3205 | This study |
h- ura4-D18 tuf1-mRFP::ura4 hph::P3nmt1:FLAG-atg43ΔN20
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3317 | This study |
h- ura4-D18 tuf1-mRFP::ura4 hph::P3nmt1:FLAG-atg43ΔN40
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3651 | This study |
h- ura4-D18 tuf1-mRFP::ura4 atg43ΔAIM::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) |
TFSP3979 | This study |
h- hph::P3nmt1:FLAG-atg43 kan::P3nmt1:GFP-atg8
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3981 | This study |
h- hph::P3nmt1:FLAG-atg43ΔAIM kan::P3nmt1:GFP-atg8
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP2721 | This study |
h- ura4-D18 tuf1-mRFP::ura4 sdh2-mEGFP::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3291 | This study |
h- ura4-D18 tuf1-mRFP::ura4 sdh2-mEGFP::hph atg43ΔN41-80::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3293 | This study |
h- ura4-D18 tuf1-mRFP::ura4 sdh2-mEGFP::hph atg43ΔN81-120::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3307 | This study |
h- ura4-D18 tuf1-mRFP::ura4 sdh2-mEGFP::hph atg43ΔN121-164::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3289 | This study |
h- atg43ΔN164::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3315 | This study |
h- atg43ΔN184::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1107 | This study |
h- atg43Δ::kan
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3261 | This study |
h- ura4-D18 tuf1-mRFP::ura4 pNATZA31-atg43(C80) hph::P81nmt1:GFP-atg43ΔN164
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP4041 | This study |
h- hph::P3nmt1:FLAG-atg43 pNATZA1-GFP
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3983 | This study |
h- hph::P3nmt1:FLAG-atg43 pNATZA1-GFP-atg8
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP4263 | This study |
h- hph::P3nmt1:FLAG-atg43 pNATZA1-GFP-atg11
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP4265 | This study |
h- hph::P3nmt1:FLAG-atg43 pNATZA1-atg11-GFP
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1461 | This study |
h- atg43-1::kanMX4-FLAG::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1021 | This study |
h- atg43-FLAG::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP4289 | This study |
h- tuf1-mRFP::nat atg43-1::kanMX4-FLAG::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3263 | This study |
h- ura4-D18 tuf1-mRFP::ura4 pNATZA31-atg43(C80) hph::P81nmt1:GFP-atg43ΔN184
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3223 | This study |
h- ura4-D18 tuf1-mRFP::ura4 sdh2-mEGFP::hph pNATZA31-atg43(C80)
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3241 | This study |
h- ura4-D18 tuf1-mRFP::ura4 sdh2-mEGFP::hph pNATZA31-atg43(C80) atg43ΔC20::kan
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3253 | This study |
h- ura4-D18 tuf1-mRFP::ura4 sdh2-mEGFP::hph pNATZA31-atg43(C80) atg43ΔC60::kan
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3305 | This study |
h- ura4-D18 tuf1-mRFP::ura4 sdh2-mEGFP::hph pNATZA31-atg43(C80) atg43ΔC61-80::kan
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP2419 | This study |
h- atg43ΔC20::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3287 | This study |
h- atg43ΔC60::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3363 | This study |
h- atg43ΔC61-80::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3225 | This study |
h- ura4-D18 tuf1-mRFP::ura4 hph::P81nmt:GFP-atg43 pNATZA31-atg43(C80)
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3255 | This study |
h- ura4-D18 tuf1-mRFP::ura4 hph::P81nmt:GFP-atg43ΔC20::kan pNATZA31-atg43(C80)
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3259 | This study |
h- ura4-D18 tuf1-mRFP::ura4 hph::P81nmt:GFP-atg43ΔC60::kan pNATZA31-atg43(C80)
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3343 | This study |
h- ura4-D18 tuf1-mRFP::ura4 hph::P81nmt:GFP-atg43ΔC61-80::kan pNATZA31-atg43(C80)
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3559 | This study |
h- leu1-32 ura4-D18 tuf1-mRFP::ura4 pNATZA31-atg43(C80)::leu1MX4 atg43ΔC60-mEGFP::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3529 | This study |
h- leu1-32 ura4-D18 tuf1-mRFP::ura4 pNATZA31-atg43(C80)::leu1MX4 kan::P3nmt1:GBP-fis1 atg43ΔC60-mEGFP::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3453 | This study |
h- leu1-32 ura4-D18 tuf1-mRFP::ura4 pNATZA31-atg43(C80)::leu1MX4
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3557 | This study |
h- leu1-32 ura4-D18 tuf1-mRFP::ura4 pNATZA31-atg43(C80)::leu1MX4 kan::P3nmt1:GBP-fis1 atg43ΔC60::hph
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3803 | This study |
h- ura4-D18 tuf1-mRFP::ura4 atg43-1::kanMX4 hph::P3nmt1:GBP-fis1 pNATZA1-GFP
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3793 | This study |
h- ura4-D18 tuf1-mRFP::ura4 atg43-1::kanMX4 hph::P3nmt1:GBP-fis1pNATZA1-GFP-atg43(N21-40)
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3785 | This study |
h- ura4-D18 tuf1-mRFP::ura4 atg43-1::kanMX4 hph::P3nmt1:GBP-fis1 pNATZA1-GFP-atg8
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3165 | This study |
h- kan::P3nmt1:GFP-mim2
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3163 | This study |
h- kan::P3nmt1:GFP-mim2 hph::P3nmt1:FLAG-atg43
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3215 | This study |
h- kan::P3nmt1:GFP-mim1
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3213 | This study |
h- kan::P3nmt1:GFP-mim1 hph::P3nmt1:FLAG-atg43
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3247 | This study |
h- ura4-D18 tuf1-mRFP::ura4 hph::P81nmt:GFP-atg43 mim1Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3173 | This study |
h- ura4-D18 tuf1-mRFP::ura4 hph::P81nmt:GFP-atg43 mim2Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3283 | This study |
h- ura4-D18 tuf1-mRFP::ura4 mim1Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3271 | This study |
h- ura4-D18 tuf1-mRFP::ura4 mim2Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1779 | This study |
h- ura4-D18 tuf1-mRFP::ura4 hph::P81nmt:GFP-atg43 tom70Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3565 | This study |
h- leu1-32 ura4-D18 tuf1-mRFP::ura4 pNATZA31-atg43(C80)::leu1MX4 kan::P3nmt1:GBP-fis1 atg43ΔC60-mEGFP::hph mim1Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3567 | This study |
h- leu1-32 ura4-D18 tuf1-mRFP::ura4 pNATZA31-atg43(C80)::leu1MX4 kan::P3nmt1:GBP-fis1 atg43ΔC60-mEGFP::hph mim2Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3569 | This study |
h- leu1-32 ura4-D18 tuf1-mRFP::ura4 pNATZA31-atg43(C80)::leu1MX4 kan::P3nmt1:GBP-fis1 atg43ΔC60-mEGFP::hph tom70Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3217 | This study |
h- kan::P3nmt1:GFP-mim2hph::P3nmt1:FLAG-mim1
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3761 | This study |
h- mim1Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3763 | This study |
h- mim2Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3161 | This study |
h- ura4-D18 tuf1-mRFP::ura4 tom70-mEGFP::kan
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3459 | This study |
h- ura4-D18 tuf1-mRFP::ura4 tom70-mEGFP::kan mim1Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3455 | This study |
h- ura4-D18 tuf1-mRFP::ura4 tom70-mEGFP::kan mim2Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3479 | This study |
h- ura4-D18 tuf1-mRFP::ura4 kan::P3nmt1:GFP-mim1
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3523 | This study |
h- ura4-D18 tuf1-mRFP::ura4 kan::P3nmt1:GFP-mim1 atg43Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3563 | This study |
h- ura4-D18 tuf1-mRFP::ura4 kan::P3nmt1:GFP-mim1tom70Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3175 | This study |
h- ura4-D18 tuf1-mRFP::ura4 kan::P3nmt1:GFP-mim2
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3525 | This study |
h- ura4-D18 tuf1-mRFP::ura4 kan::P3nmt1:GFP-mim2 atg43Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3561 | This study |
h- ura4-D18 tuf1-mRFP::ura4 kan::P3nmt1:GFP-mim2 tom70Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3485 | This study |
h- kan::P3nmt1:GFP-mim2 pNATZA31-atg43(C80) hph::P3nmt1:FLAG-atg43
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3487 | This study |
h- kan::P3nmt1:GFP-mim2 pNATZA31-atg43(C80) hph::P3nmt1:FLAG-atg43ΔN164
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3489 | This study |
h- kan::P3nmt1:GFP-mim2 pNATZA31-atg43(C80) hph::P3nmt1:FLAG-atg43ΔN184
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3601 | This study |
h- leu1-32 hph::P3nmt1:GFP-mim2 pNATZA31-atg43(C80) leu1MX4::P3nmt1:FLAG-atg43ΔC61-80::kan
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3617 | This study |
h- leu1-32 hph::P3nmt1:GFP-mim2 pNATZA31-atg43(C80) leu1MX4::P3nmt1:FLAG-atg43ΔC20::kan
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3603 | This study |
h- leu1-32 hph::P3nmt1:GFP-mim2 pNATZA31-atg43(C80) leu1MX4::P3nmt1:FLAG-atg43ΔC60::kan
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3201 | This study |
h- tom70-FLAG::hph kan::P3nmt1:GFP-mim2
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3233 | This study |
h- hph::P3nmt1:FLAG-atg43 tom70-mEGFP::kan
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP3649 | This study |
h- atg43ΔAIM::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | MKY1 | NBPR (https://yeast.nig.ac.jp/yeast/) | FY7455 | h+ leu1-32 ura4 his7 lys1 |
| Strain, strain background (S. pombe) | TFSP3481 | This study |
h+ leu1-32 ura4 his7 lys1 atg7Δ::kan
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP4259 | This study |
h+ leu1-32 ura4 his7 lys1 atg43ΔAIM::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP1559 | This study |
h- isp6Δ::hph atg7Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP4255 | This study |
h- isp6Δ::hph atg43ΔAIM::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP10 | This study |
h- sdh2-mEFGP::kan
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP4271 | This study |
h- sdh2-mEFGP::kan atg7Δ::nat
Stocked in T. Kanki lab. |
|
| Strain, strain background (S. pombe) | TFSP4273 | This study |
h- sdh2-mEFGP::kan atg43ΔAIM::nat
Stocked in T. Kanki lab. |
|
| Antibody | anti-RFP (Rabbit polyclonal) | MBL | Cat# PM005, RRID:AB_591279 | WB (1:3000) |
| Antibody | anti-GFP (Mouse monoclonal) | Takara Bio | Cat# 632380, RRID:AB_10013427 | WB (1:10000) |
| Antibody | anti-histone H3 (Rabbit polyclonal) | Abcam | Cat# ab1791, RRID:AB_302613 | WB (1:10000) |
| Antibody | anti-FLAG (Mouse monoclonal) | Sigma | Cat# F1804, RRID:AB_262044 | WB (1:4000) |
| Antibody | anti-DDDDK (Rabbit polyclonal) | MBL | Cat# PM020, RRID:AB_591224 | WB (1:4000) |
| Antibody | anti-Atp2 (Rabbit polyclonal) | Abcam | Cat# ab128743, RRID:AB_2810299 | WB (1:2000) |
| Antibody | anti-Actin (Mouse monoclonal) | Abcam | Cat# ab8224, RRID:AB_449644 | WB (1:4000) |
| Antibody | anti-mouse IgG (Goat polyclonal, Peroxidase conjugated) | Merck Millipore | Cat# AP124P, RRID:AB_90456 | WB (1:10000) |
| Antibody | anti-rabbit IgG (Goat polyclonal, Peroxidase conjugated) | Jackson ImmunoResearch | Cat# 111-035-003, RRID:AB_2313567 | WB (1:10000) |
| Antibody | anti-guineapig IgG (Goat polyclonal, Peroxidase conjugated) | Jackson ImmunoResearch | Cat# 106-035-003, RRID:AB_2337402 | WB (1:10000) |
| Antibody | anti-FLAG beads | Sigma | Cat# M8823, RRID:AB_2637089 | |
| Antibody | anti-GFP beads | Chromotek | Cat# gtma, RRID:AB_2631358 | |
| Antibody | anti-Atg43 (Guineapig polyclonal) | This study | WB (1:4000) Stocked in T. Kanki lab. |
|
| Recombinant DNA reagent | pFA6a-leu1MX4 (plasmid) | NBPR | FYP2892 | |
| Recombinant DNA reagent | pJQW#600 (plasmid) | NBPR | FYP3955 | |
| Recombinant DNA reagent | pNATZA1 (plasmid) | NBPR | FYP2874 | |
| Recombinant DNA reagent | pNATZA31 (plasmid) | NBPR | FYP2879 | |
| Recombinant DNA reagent | pNATZA31-atg43 (plasmid) | This study | Stocked in T. Kanki lab. | |
| Recombinant DNA reagent | pNATZA31-atg43(C80) (plasmid) | This study | Stocked in T. Kanki lab. | |
| Recombinant DNA reagent | pNATZA1-GFP (plasmid) | This study | Stocked in T. Kanki lab. | |
| Recombinant DNA reagent | pNATZA1-GFP-atg8 (plasmid) | This study | Stocked in T. Kanki lab. | |
| Recombinant DNA reagent | pNATZA1-GFP-atg11 (plasmid) | This study | Stocked in T. Kanki lab. | |
| Recombinant DNA reagent | pNATZA1-atg11-GFP (plasmid) | This study | Stocked in T. Kanki lab. | |
| Recombinant DNA reagent | pNATZA1-GFP-atg43(N21-40) (plasmid) | This study | Stocked in T. Kanki lab. | |
| Commercial assay or kit | EzWestLumi One | Atto | WSE-7110 | |
| Commercial assay or kit | EzWestLumi plus | Atto | WSE-7120 | |
| Commercial assay or kit | Clarity Max Western ECL Substrate | Bio-Rad | 1705062 | |
| Chemical compound, drug | MitoTracker Red CMXRos | Thermo Fisher Scientific | M7512 | (250 nM) |
| Chemical compound, drug | Mito SOX Red | Thermo Fisher Scientific | M36008 | (5 µM) |
| Software, algorithm | Image Lab | Bio-Rad | ||
| Software, algorithm | MetaMorph 7 | Molecular Devices |