Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) |
C57BL/6 | Charles Rivers | ||
| Genetic reagent (Mus musculus) |
Mmp14f/f | Gutiérrez-Fernández et al., 2015 | MGI:5694577 | Dr. Carlos López-Otín |
| Genetic reagent (Mus musculus) |
Lyz2-Cre | Clausen et al., 1999 | MGI:1934631 | |
| Genetic reagent (Mus musculus) |
Cdh5-CreERT2 | Sörensen et al., 2009 | MGI:3848982 | |
| Genetic reagent (Mus musculus) |
R26TdTomato | Madisen et al., 2010 | MGI:3809524 | |
| Cell line (Homo sapiens) |
HEK293 | Sigma | ||
| Antibody | Anti-CD16/CD32 (rat monoclonal) |
BD Biosciences | 553141 | (1:100) |
| Antibody | Anti-CD45 (rat monoclonal) | Biolegend | 103132 | (1:100) |
| Antibody | Anti-CD45 (rat monoclonal) | Biolegend | 103116 | (1:100) |
| Antibody | Anti-CD45 (rat monoclonal) | eBioscience | 48–0451 | (1:100) |
| Antibody | Anti-CD11b (rat monoclonal) | BD Biosciences | 552850 | (1:100) |
| Antibody | Anti-CD11b (rat monoclonal) | BD Biosciences | 557395 | (1:100) |
| Antibody | Anti-CD11b (rat monoclonal) | Biolegend | 101206 | (1:100) |
| Antibody | Anti-Ly6C (rat monoclonal) | BD Biosciences | 560595 | (2:100) |
| Antibody | Anti-Ly6C (rat monoclonal) | BD Biosciences | 553104 | (1:100) |
| Antibody | Anti-CD31 (rat monoclonal) | BD Biosciences | 551262 | (1:100) |
| Antibody | Anti-CD31 (rat monoclonal) | BD Biosciences | 553372 | (1:100) |
| Antibody | Anti-PDGFR-β (rat monoclonal) | BioLegend | 136005 | (2:100) |
| Antibody | Anti-PDGFR-β (rat monoclonal) | BioLegend | 136007 | (2:100) |
| Antibody | Anti-Feeder Cells (MEFSK4) (rat monoclonal) |
Miltenyi | 130-120-166 | (2:100) |
| Antibody | Anti-Feeder Cells (MEFSK4) (rat monoclonal) |
Miltenyi | 130-120-802 | (2:100) |
| Antibody | Anti-F4/80 (rat monoclonal) | BioLegend | 123114 | (3:100) |
| Antibody | Anti-F4/80 (rat monoclonal) | BioLegend | 123110 | (3:100) |
| Antibody | Anti-Phospho-Smad2 (rabbit polyclonal) |
Cell Signaling | 3104 | (1:100) |
| Antibody | Streptavidin-Alexa 488 conjugate |
ThermoFisher Scientific | S11223 | (1:500) |
| Antibody | Anti-LAP (mouse monoclonal) |
Biolegend | 141405 | (3:100) |
| Antibody | Anti-TGFβ1 (rabbit polyclonal) |
Abcam | ab92486 | (1:100) |
| Antibody | anti-rabbit IgG (chicken polyclonal) |
ThermoFisher | A-21441 | (1:500) |
| Antibody | anti- rabbit IgG (goat polyclonal) |
ThermoFisher | A-21245 | (1:500) |
| Antibody | anti-CAI-IX (rabbit polyclonal) |
Abcam | ab15086 | (1:100) |
| Antibody | anti-rabbit IgG (goat polyclonal) |
ThermoFisher | A-11035 | (1:500) |
| Antibody | anti-CD31 (rat monoclonal) |
Dianova | DIA-310 | (1:200) |
| Antibody | anti-Rat IgG (goat polyclonal) |
ThermoFisher | A-11006 | (1:500) |
| Antibody | anti-pSMAD2 (rabbit monoclonal) |
Cell Signaling | 3108 | (1:100) |
| Antibody | anti-SMA (mouse monoclonal) |
Sigma-Aldrich | C6198 | (1:400) |
| Antibody | anti-ERG (rabbit monoclonal) |
Abcam | ab196149 | (1:100) |
| Antibody | Anti-PDGFRβ (rat monoclonal) |
Thermofisher | 14-1402-82 | (1:100) |
| Antibody | Anti-rat IgG (goat polyclonal) |
Thermofisher | A-11006 | (1:500) |
| Antibody | Anti-TGFβ1 (rabbit polyclonal) |
Santa Cruz | sc-146 | (1:500) |
| Antibody | Anti-TfR (rabbit polyclonal) |
Abcam | ab84036 | (1:1000) |
| Antibody | Anti-α-tubulin (mouse monoclonal) |
Sigma | T6074 | (1:1000) |
| Antibody | Anti-TGFβ1 (mouse monoclonal) |
inVivoMab/Bio X Cell | BE0057 | 100 µg/mL |
| Antibody | anti-ICAM2 (rat monoclonal) |
BD Biosciences | 553325 | (1:500) |
| Antibody | anti-rabbit IgG (goat polyclonal) |
Jackson | 111-035-003 | (1:7500) |
| Antibody | anti-mouse IgG (goat polyclona) |
Jackson | 115-035-003 | (1:7500) |
| Recombinant DNA reagent | p3TP-lux | Wrana et al., 1992 | RRID:Addgene_11767 | Dr. Carmelo Bernabeu |
| Sequence-based reagent | qPCR primers | This paper | Supplement file 1 | |
| Peptide, recombinant protein | Human TFGβ1 | Peprotech | 100–21 | |
| Peptide, recombinant protein | IL4 | Peprotech | 214–14 | |
| Peptide, recombinant protein | Murine M-CSF | Peprotech | 315–02 | |
| Chemical compound, drug | LPS | Sigma | L2654 | |
| Chemical compound, drug | Tamoxifen | Sigma | T5648 | |
| Chemical compound, drug | Collagenase type IV | Sigma | C5138 | |
| Chemical compound, drug | Collagenase type I | Worthington | LS004194 | |
| Chemical compound, drug | Collagenase type II | Worthington | LS004174 | |
| Chemical compound, drug | Porcine pancreatin | Sigma | P3292 | |
| Chemical compound, drug | RBC Lysis buffer solution | eBioscience | 00-4333-57 | |
| Chemical compound, drug | Passive Lysis 5× Buffer | Promega | E1941 | |
| Chemical compound, drug | TRIzol Reagent | Thermofisher | 15596026 | |
| Chemical compound, drug | Fluoromont-G | Southern Biotech | 0100–01 | |
| Commercial assay or kit | Dynabeads Sheep Anti-Rat IgG |
Thermofisher | 11035 | |
| Commercial assay, kit | Foxp3/Transcription Factor Staining Buffer Set |
eBioscience | 00-5523-00 | |
| Commercial assay, kit | High Capacity cDNA Reverse Transcription Kit |
Applied Biosystems | 4368814 | |
| Commercial assay, kit | Bradford Assay | Bio-Rad | 5000001 | |
| Commercial assay, kit | Luciferase Assay System | Promega | PR-E1500 | |
| Software, algorithm | Vevo 2100 | Visual Sonics | RRID:SCR_015816 | |
| Software, algorithm | Fiji | fiji.sc | RRID:SCR_002285 | |
| Software, algorithm | GraphPad Prism | www.graphpad.com | RRID:SCR_002798 | |
| Software, algorithm | FlowJo | www.flowjo.com | RRID:SCR_008520 | |
| Software, algorithm | qBASE+ (Biogazelle) | www.qbaseplus.com | RRID:SCR_003370 | |
| Software, algorithm | NDP.view2 | Hamamatsu Photonics | ||
| Software, algorithm | Zen2 | Zeiss | RRID:SCR_013672 | |
| Software, algorithm | 3D fully automated image analysis |
Gkontra et al., 2018 |