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. 2020 Oct 21;9:e60853. doi: 10.7554/eLife.60853

Figure 2. The effects of yeast deprivation on male sleep develop over multiple days but can be reversed within a few hours.

Figure 2.

(A) Schematic diagram of the experimental design for (B) and (C). Normally fed MF pairs were loaded into tubes containing 5% sucrose or normal food between ZT 1–2, and sleep was assayed for six nights, starting at ZT 12 on the loading day. (B) Sleep profile in 30 min intervals for normally fed MF pairs loaded into tubes containing either 5% sucrose food or normal food. Flies were transferred to new tubes after 3 days to circumvent the potential problem of larval movements interfering with sleep measurements. The rectangle and arrow indicate the time of transfer. N = 59–60. (C) Nighttime sleep for the flies shown in (B) Time from loading until the begining of each night is indicated in parentheses. (D) Schematic diagram of the experimental design for (E), (F) and (G). After 6 days in the indicated nutritional conditions, MF pairs were loaded into tubes containing either 5% sucrose or normal food. (E) Sleep profile in 30 min intervals for MF pairs in the normal food condition loaded into tubes containing either 5% sucrose food or normal food. (F) Sleep profile in 30 min intervals for MF pairs in the yeast deprivation condition loaded into tubes containing either 5% sucrose food or normal food. N = 31–32. (G) Nighttime sleep for the flies shown in (E) and (F). (H) Schematic diagram of the experimental design for (I) and (J). MF pairs yeast-deprived for 6 days were loaded into tubes containing 5% sucrose. Flies were transferred to tubes containing either 5% sucrose or normal food at ZT 12 on the following day. (I) Sleep profile in 30 min intervals for yeast-deprived MF pairs transferred into tubes containing either 5% sucrose food or normal food. The rectangle and arrow indicate the time of transfer. N = 51–54. (J) Nighttime sleep for the flies shown in (I). Iso31 flies were used in all panels. ****p<0.0001 and ns: not significant, two-way ANOVA followed by Sidak post hoc test (C), one-way ANOVA followed by Tukey post hoc test (G), unpaired t-test (J).