Figure 5. Dopaminergic neurons projecting to the protocerebral bridge (DA-PB) act downstream of male-specific P1 neurons.
(A) Confocal projection of an adult male brain in which Syt::GFP was driven by P1-spG4. Anti-Bruchpilot (BRP, magenta) was used to localize neuropil regions. The image on the right shows a magnification of the PB region, which contains presynaptic terminals from P1 neurons. (B) Confocal projection of an adult male brain in which DenMark (postsynaptic marker, left) and Syt::GFP (presynaptic marker, right) are expressed in DA-PB neurons. Both postsynaptic and presynaptic makers are expressed in the PB region. (C) Increase in GCaMP6m signal (ΔF) in the PB projections of DA-PB neurons upon perfusion with ATP of a male brain expressing P2X2 in P1 neurons (P1 >P2X2, top right) or a genetic control (P2X2/+, top left). Fluorescence traces (bottom left) and peak responses (bottom right) for normalized GCaMP6m response (ΔF/F0) in the PB projections of DA-PB neurons in response to P1 activation (blue, P1 >P2X2) compared with the genetic control (black, P2X2/+). R71G01-lexA was used to express P2X2 in P1 neurons and SS52578 spG4 was used to express GCaMP6m in DA-PB neurons. Grey rectangle indicates 2.5 mM ATP perfusion. N = 14–19. Scale bars represent 50 μm in (A–B) and 10 μm in (C). **p<0.01, unpaired t-test with Welch’s correction for unequal variances.
Figure 5—figure supplement 1. Female DA-PB neurons show similar projection patterns as male counterparts.
