Table 2.
Effect ILs toward cytokines, cytolitic enzyme of co-culture breast cancer and NK cells
| Samples (Co-culture MCF7+NK) in various ratio | Level of cytokines, cytolitic enzyme | |||
|---|---|---|---|---|
| IFN-γ (pg/ml) | TNF-α (pg/ml) | PRF1 (pg/ml) | GzmB (pg/ml) | |
| Control (MCF7) | 1.83±0.33 a | 1.98±0.24 a | 20.00±10.00 a | 13.33±4.51a |
| MCF7 + NK(1:1) | 14.50±3.93 b | 5.24±0.28 a | 40.00±10.00 ab | 29.00±4.36 b |
| MCF7 + NK(1:5) | 32.28±1.35 c | 13.85±2.79 b | 110.00±10.00 b | 55.67±12.50 b |
| MCF7 + IL2-NK(1:1) | 17,39±3.56 b | 15.04±0.21 bc | 160.00±40.00 cd | 87.00±11.79 c |
| MCF7 + IL2-NK (1:5) | 35.72±0.38 c | 37.44±1.47 d | 230.00±40.00 de | 149.00±7.21 d |
| MCF7 + IL15-NK (1:1) | 18.28±5.74 b | 19.03±1.66 bc | 150.00±20.00 cd | 88.67±11.68 c |
| MCF7 + IL15-NK (1:5) | 59.28±1,90 d | 42.21±4.23 d | 250.00±30.00de | 140.67±12.86 d |
| MCF7 + IL18-NK (1:1) | 17.72±3.37 b | 20.44±2.37 c | 170.00±40.00 cd | 96.00±1.73 c |
| MCF7 + IL18-NK (1:5) | 60.17±3.71 d | 60.56±3.15 e | 320.00±70.00 f | 149.67±11.93 d |
*This research was peroformed 3 replication for IFN-γ, TNF-α, PRF1, GzmB. Data are presented as mean ±standard deviation. Different supercript small letters (a,b,c,d) in the same coloumn (among ratio of MCF7 and ILs-induced NK cells in IFN-γ level), different supercript small letters (a,bc,c,d) in the same coloumn (among ratio of MCF7 and ILs-induced NK cells in TNF-α level), different supercript small letters (a,ab,b, cd,de,f) in the same coloumn (among ratio of MCF7 and ILs-induced NK cells in PFR1 level), different supercript small letters (a,b,c,d) in the same coloumn (among ratio of MCF7 and ILs-induced NK cells in GzmB level), were significantly different at p < 0.05 (Tukey honestly significant difference post hoc test).