Table 1.
Primers for genotyping
Name | Sequences | |
---|---|---|
Efnb2 | WT | Forward: 5′- GCTGCCCGCGGCCGGTCCCAACG-3′ Reverse: 5′- CCGTTAGTGGCAACGTCCTCCGTC CTC-3′ |
Floxed allele | Forward: 5′-AAGTTATAAGCT TCAACGCGTCC-3′ Reverse: 5′- GAGCCCCAGGTTCTAGAATAA-3′ |
|
Cre | Forward: 5′-GCAAAACAG GCTCTAGCGTTCG-3′ Reverse: 5′-CTGTTTCACTATCCAGGTTACGG-3′ |
|
tdT | WT | Forward: 5′-AAGGGAGCTGCAGTGGAGTA-3′ Reverse: 5′-CCGAAA ATCTGTGGGAGGTC-3′ |
Transgene | Forward: 5′-CTGTTCCTGTACGGCATGG-3′ Reverse: 5′-GGCATTAAAGCAGCGTATCC-3′ |
Abbreviations: Efn2, ephrin b2; tdT, tdTomato; WT, wild-type.