Table 1. Three pairs of forward and reverse primers used for SARS-CoV2 spike gene PCR amplification.
| Forward and reverse primers | ||
|---|---|---|
| 3,822 bp | ||
| CoV2F-1 | 5′ ATGTTTGTTTTTCTTGTTTTATTGC | |
| CoV2R-1 | 5′ TTATGTGTAATGTAATTTGACTCC | |
| 719 bp | ||
| CoV2F-2 | 5′ AACAATCTTGATTCTAAGGTTGGTGG | |
| CoV2R-2 | 5′ GTCTGAGTCTGATAACTAGCG | |
| 2,010 bp | ||
| CoV2F-3 | 5′ GCCACTAGTCTCTAGTCAGTG | |
| CoV2R-3 | 5′ CCGCCGAGGAGAATTAGTCT | |
The forward and reverse primer sequence were shown in three panels. The expected PCR product size of SARS-CoV2 spike gene was indicated on the top of each panel.