Fig. 2.

Generation of CMIP transgenic mice. a Schematic diagram of targeted transgenesis into the HPRT locus (see details in the Materials and Methods section). All mice analyzed in this study were hemizygous males from the F10 to F18 generations. b PCR genotyping of mouse tail DNA. The HPRT PCR product was amplified from the WT allele in homozygous transgenic females (Tg+/+) or hemizygous transgenic males (Tg+) but not the reconstituted HPRT gene. c Quantification of mouse and human CMIP transcripts in transgenic mice and WT mice by RT-qPCR. d Confocal immunofluorescence microscopy of CMIP expression in T cells from WT and transgenic mice. The rightmost figure in the panel corresponds to the negative control, in which IgG isotype control antibody was used instead of primary antibody (Cmip/CD4) in Tg T cells