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mRNA injection time determines distribution of fluorophore. a Microinjection at very early embryo stage (1–4 cell) generates homogenous expression of fluorophore across entire embryo, while later stage injections (16–32 cell) generates confined/mosaic patterning of fluorophore. b Depending on time of injection, patterning on embryo can be imaged with FCS or with FCCS using two or more fluorophores
