Figure 3.

Kinetic analysis of RAP interaction with immobilized full-length LRP1 and clusters II, III and IV. SDS-PAGE of RAP in reducing conditions, molecular weight markers are indicated in kDa (center panel). SPR analysis of RAP interaction with full-length LRP1: RAP was injected at indicated concentrations over immobilized full-length LRP1 (A, 7,400 RU), and LRP1 clusters II (B, 2,967 RU), III (D, 3,460 RU) and IV (C, 3,396 RU) in 50 mM triethanolamine-HCl (TEA), 145 mM NaCl, 1 mM CaCl₂, 0.005% surfactant P20, pH 7.4. The fitting obtained using a Langmuir 1:1 binding model is shown in black lines. Kinetic constants are summarized in Table 1.