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Kinetic parameters of Glucosylceramidase in different concentrations of gluconolactone and glucose.
| I0 |
I1 |
I2 |
Conclusion |
|
|---|---|---|---|---|
| Gluconolactone | ||||
| Vmax and Vmaxapp | 333.3 | 333.3 | 333.3 | Vmax unchanged |
| Km and Kmapp | 3.6 | 5.6 | 8.6 | The apparent Km increased |
| Ki | 0.023 | 0.119 | Competitive Inhibition | |
|
Glucose | ||||
| Vmax and Vmaxapp | 333.3 | 90.9 | 13.5 | Vmax decreased |
| Km and Kmapp | 8.9 | 2.6 | 0.27 | The apparent Km decreased |
| Ki | 2.09 | 1.94 | Uncompetitive Inhibition | |
