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Effect of 6-OHDA on intracellular ROS levels. SH-SY5Y cells were incubated with 20 μM 6-OHDA for 0, 3, 6, 9, 18 or 24 h. The results represent the means ± SD 6 experiments. ∗p < 0.05, ∗∗p < 0.01 compared to control of same incubation time.