Fig. 1.

(A) Top, representative microphotographs of vehicle- (Veh) and AK7-treated spinal organotypic cultures (SOCs) embedded in collagen and immunolabeled to reveal neurofilaments using the RT97 antibody. Bottom, graphs histogram showing the number of neurites per intersection, and bar graph indicating the maximum neurite length (n = 11–12, ANOVA, post hoc Bonferroni, *P < 0.05 vs. Veh). Scale bar = 250 μm and 50 μm (images with high magnification). (B) Representative confocal images of motoneurons (MNs) (arrows) from SOCs of different conditions immunostained against GAP43 (green) and counterstained with RT-97 (red). Scale bar = 25 μm. Bar graph of the mean (± SEM) intensity of GAP43 immunoreactivity inside the cytoplasm of MNs (n = 4 SOCs slices, t test *P < 0.05)