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. 2020 Nov 3;10:18946. doi: 10.1038/s41598-020-75873-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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UVB-induced early cell death in primary human fibroblasts. Using Annexin V-FITC and PI labelling, we assessed cell death in fibroblasts at 3 h and 6 h following an irradiation with 20 kJ/m² UVB. The fluorescence signal in each cell was assessed by FACS. (a) Total cell death is the addition of Annexin V+/PI− cells, Annexin V+/PI+ cells and Annexin V−/PI+ cells. (b) PI positive cells is composed of PI+/Annexin V+ or − cells. (c) Annexin V positive cells are Annexin V+ and PI−. NoUV are unirradiated control cells. UVB irradiation of fibroblasts leads to cell death as early as 3 h. Percentage is representative of a population of at least 8000 cells. N = 3, *p-value < 0.05.