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. 2020 Nov 3;10:18946. doi: 10.1038/s41598-020-75873-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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UVB induces two independent reduction of cellular metabolic activity in primary human fibroblasts. Prior to UVB irradiation, fibroblasts were incubated 30 min with different combinations of cell death inhibitors. Cells were then irradiated in PBS using a lethal UVB dose (30 kJ/m²). The combinations used were: (a) Necroptosis inhibitor Necrosulfonamide (NSA, 2 μM) and caspase inhibitor Q-VD-OPh (QVD, 20 μM), (b) ferroptosis inhibitor Ferrostatin-1 (Ferro-1, 5 μM) and caspase inhibitor Q-VD-OPh (QVD, 20 μM), (c) PARP inhibitor ABT888 (ABT, 20 μM) and caspase inhibitor Q-VD-OPh (QVD, 20 μM), (d) PARP inhibitor 3-Aminobenzamide (3-ABA, 0.5 mM) and caspase inhibitor Q-VD-OPh (QVD, 20 μM). Cellular metabolic activity was assessed at different time points post UVB irradiation (0, 1, 3, 6, 24 h) using MTS assay. Irradiated cells were normalised on unirradiated cells of the same condition. NSA and Ferro-1 had no effect on cellular metabolic activity loss prevention caused by QVD. However, an additive effect of QVD and PARP inhibitors (ABT and 3-ABA) on prevention of cellular metabolic activity loss caused by UVB could be described. N = 4. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001.