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. 2020 Nov 3;10:18953. doi: 10.1038/s41598-020-76151-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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S. purpurea reduced HSV-1 ICP4, ICP8, and gC protein levels in a time dependent manner. Vero cells were mock infected or infected with HSV-1 at a MOI = 5 in the presence or absence of S. purpurea (40 µg/ml) added at 0, 1, 2, 4, and 6 h.p.i. Cells were harvested at 16 h.p.i., lysed, separated by SDS-PAGE analyzed by Western blot with antibodies to HSV-1 ICP4, ICP8, gC and cellular actin. Actin was included as a standard loading control. (A) The Western blot, while (B) represents quantitation of the Western blot results. Error bars indicate the standard deviation from three separate analyses. Statistical analysis was performed using a paired t-test. Samples with statistically significant deviation relative to the untreated HSV-1 sample are indicated with asterisks (*p < 0.05, **p < 0.01, ***p < 0.005). Images of the full-length Western blots are included in the “Supplemental files”.