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. 2020 Nov 3;10:18953. doi: 10.1038/s41598-020-76151-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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S. purpurea inhibited HSV-1 ICP4, ICP8, and gC gene expression. Vero cells were mock infected or infected with HSV-1 at a MOI = 5 in the presence or absence of S. purpurea (40 µg/ml) added at 0, 1, 2, 4, and 6 h.p.i. Cells were harvested at 8 h.p.i. and total RNA was isolated. The level of HSV-1 ICP4, ICP8, and gC gene expression was analyzed by real-time PCR. Cellular GAPDH was used as an internal reference and normalization. Error bars indicate the standard deviation from three separate trials. Statistical analysis was performed using a paired t-test. Samples with statistically significant deviation relative to the untreated HSV-1 sample are indicated with asterisks (*p < 0.05, **p < 0.01, ***p < 0.005).