Fig. 3.

LXRs agonist GW3965 reversed FS2-elicited anxiety-like behaviors. GW3965 (GW, 10 mg/kg) was administered by intraperitoneal (i.p.) injection once a day before acute stress-induced by forced swimming twice (one 30 min-swim per day, for 2 days, FS2), then the effects of GW on anxiety-like behaviors were determined by elevated plus maze test (EPM) and open field test (OFT). (a) The chemical structure of GW3965. (b) The time spent in the open arms, (c) the entries in the open arms, and (d) the total arm entries in the EPM test from each group. (e) The time spent in the central area and (f) the total distance traveled in OFT. GW administration alleviated FS2-stressed mice anxiety-like behaviors but had no effect on control mice. Each value represented the mean ± SEM of three independent experiments (n = 6 experiments, **p < 0.01, vs control group; ^#p <  0.05, vs FS2-stressed group)