Fig. 5.

Knockdown of LXRβ expression in the amygdala by shLXRβ blocked GW3965-mediated anxiolytic effects in FS2-stressed mice. Mice were infected with LXRβ-shRNA (shLXRβ) or negative shRNA (shNC) in the amygdala for 14 days, followed by forced swimming twice (one 30 min-swim per day, for 2 days, FS2) with or without GW3965 (GW, 10 mg/kg, i.p.) administration, then the effects of GW on anxiety-like behaviors were determined by elevated plus maze test (EPM) and open field test (OFT). (a) The time spent in the open arms, (b) the entries in the open arms, and (c) the total arm entries in the EPM test from each group. (d) The time spent in the central area and (e) the total distance traveled in OFT. Each value represented the mean ± SEM of three independent experiments (n = 6, **p < 0.01, vs shNC-injected group; ^##p < 0.01, vs shNC+FS2 group; ^&p < 0.05, vs shLXRβ group; ^$$p < 0.01, vs shNC+FS2+GW group)