Fig. 5.
SMAP (DBK-1160) inhibits HHcy-induced Aβ overproduction in rats. a Western blots for APP, sAPPβ, pT668APP, BACE1, and PS1 in rat hippocampus. b–f Quantification of the relative protein levels (APP, sAPPβ, pT668APP, BACE1, and PS1) after normalization to the GAPDH signal. g, h IP blots and quantitatively analysis of BACE1 interacted with APP in rat hippocampus. P: preventive group, treated with homocysteine and DBK-1160 simultaneously; 1160: treatment group, treated with DBK-1160 14 days after homocysteine injection; SCR: positive control group, treated with SCR-1693 14 days after homocysteine injection. i BACE activity assay in different groups in rat hippocampus. j, k ELISA assay of the levels of Aβ40 and Aβ42 in the rat hippocampus. Data represent mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001