Fig. 3.

Montelukast treatment and effects on microglia. (a) Iba1 immunostaining in the dentate gyrus of WT and alpha-syn mice treated with vehicle or Montelukast. Insert show representative cellular morphology. Control stainings, in which the primary goat Iba1 antibody was omitted, excluded unspecific labelling of the secondary antibody. (b) Number, and (c) cell soma size of Iba1 positive cells of the different genotype and treatment groups. (d) TSPO/Iba1 double immunofluorescence staining illustrating localization of TSPO primarily in Iba1 cells. (e) Quantitative analysis of TSPO staining intensity. (f) Correlation microglia soma size with memory. Correlations were performed by Pearson correlation. Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. One-way ANOVA was performed (b, c, e). Scale bars: (a) 30 μm, (d) 20 μm