Fig. 4.

Montelukast reduces alpha-synuclein in alpha-syn mice. (a) Human alpha-synuclein (red) and NeuN (green) immunostainings in TG mice treated with vehicle or Montelukast. Note the strong human alpha-synuclein positive accumulation in the granular cell layer of the dentate gyrus of the vehicle treated TG animals, which was hardly detected in the Montelukast treated animals. (b) Quantitative analysis of the percentage area of the staining confirmed the lower levels of alpha-syn in the Montelukast treated group. (c) Number of human alpha-syn positive neuronal cell bodies in the dentate gyrus granular cell layer. (d) Correlation %Area aSyn with memory. Correlation was performed by Pearson correlation. Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. Unpaired Student’s t test was performed (b, c). Scale bars, 25 μm