Fig. 2.

miR‐642a‐5p is involved in LPS‐induced hyperpermeability and apoptosis of PMVECs. (A) The level of miR‐642a‐5p in PMVECs transfected with NC inhibitor or miR‐642a‐5p inhibitor and stimulated by LPS (10 mg·L⁻¹, 24 h) was determined by qRT‐PCR. (B) The TER of these PMVECs transfected with NC inhibitor or miR‐642a‐5p inhibitor and stimulated by LPS (10 mg·L⁻¹, 24 h) was measured. (C) The phosphorylation level of moesin in these PMVECs transfected with NC inhibitor or miR‐642a‐5p inhibitor and stimulated by LPS (10 mg·L⁻¹, 24 h) was measured by western blot. (D) The cell viability of these PMVECs transfected with NC inhibitor or miR‐642a‐5p inhibitor and stimulated by LPS (10 mg·L⁻¹, 24 h) was detected by MTT. (E, F) The apoptosis of these PMVECs transfected with NC inhibitor or miR‐642a‐5p inhibitor and stimulated by LPS (10 mg·L⁻¹, 24 h) was assessed using the caspase‐3 activity assay and flow cytometry analysis. One‐way ANOVA and a Dunn's post hoc test were used. Experiments were repeated independently in triplicate. Data were presented as mean ± SD. **P < 0.01, ***P < 0.001.