Fig. 3.

eEF2 is a direct target of miR‐642a‐5p. (A) The prediction for miR‐642a‐5p binding sites on the 3′ UTR of eEF2 and schematic of luciferase reporter vector constructs WT‐eEF2 and the miR‐642a‐5p binding site mutated one (MUT‐eEF2). (B) Relative luciferase activities of psi‐CHECK2 carrying WT‐eEF2 or MUT‐eEF2 in PMVECs cotransfected with miR‐642a‐5p mimics or miR‐NC. (C) The relative RNA expression of miR‐642a‐5p and eEF2 in PMVECs transfected with miR‐642a‐5p mimics and miR‐642a‐5p inhibitors or their respective NCs were detected by qRT‐PCR. (D) The protein level of eEF2 in PMVECs transfected with miR‐642a‐5p mimics and miR‐642a‐5p inhibitors or their respective NCs was detected by western blot. Student's t‐test (B) or one‐way ANOVA and a Dunn's post hoc test were used (B–D). Experiments were repeated independently in triplicate. Data were presented as mean ± SD. **P < 0.01, ***P < 0.001. NS, not significant.