Fig. 5.

eEF2 inhibited LPS‐induced hyperpermeability and apoptosis of PMVECs. (A, B) The RNA and protein levels of eEF2 in PMVECs transfected with pSin‐eEF2 or empty vector and stimulated by LPS were determined by qRT‐PCR and western blot, respectively. (C) The TER of these PMVECs transfected with pSin‐eEF2 or empty vector and stimulated by LPS was measured by TER assay. (D) The phosphorylation level of moesin in these PMVECs transfected with pSin‐eEF2 or empty vector and stimulated by LPS was measured by western blot. (E) The cell viability of these PMVECs transfected with pSin‐eEF2 or empty vector and stimulated by LPS was measured by MTT. (F, G) The apoptosis of these PMVECs transfected with pSin‐eEF2 or empty vector and stimulated by LPS was measured using the caspase‐3 activity assay and flow cytometry analysis. One‐way ANOVA and a Dunn's post hoc test were used. Experiments were repeated independently in triplicate. Data were presented as mean ± SD. **P < 0.01, ***P < 0.001.