Fig. 6.

The effect of miR‐642a‐5p on the LPS‐induced hyperpermeability and apoptosis of PMVECs was rescued by eEF2 overexpression. (A, B) PMVECs cotransfected with mimics NC and empty vector (miR‐NC+vector), miR‐642a‐5p mimics and empty vector (miR‐642a‐5p+vector) or miR‐642a‐5p mimics and eEF2 overexpression plasmid (miR‐642a‐5p+eEF2), and then stimulated by LPS. The RNA and protein levels of eEF2 were measured by qRT‐PCR and western blot. (C) The TER of these cotransfected and LPS‐stimulated PMVECs was assessed by TER assay. (D) The phosphorylation level of moesin in these cotransfected and LPS‐stimulated PMVECs was determined by western blot. (E) The cell viability of these cotransfected and LPS‐stimulated PMVECs was detected by MTT. (F, G) The apoptosis of these cotransfected and LPS‐stimulated PMVECs was measured using the caspase‐3 activity assay and flow cytometry analysis. One‐way ANOVA and a Dunn's post hoc test were used. Experiments were repeated independently in triplicate. Data were presented as mean ± SD. **P < 0.01.