Fig. 1.

BACE inhibitor MBi-9 enhances axonal regeneration following a sciatic nerve crush at 2 weeks after injury. (A, B) Representative EM images of sciatic nerve cross sections from vehicle (A) and inhibitor (B) treated mice showing many more regenerated axons in the treated images (yellow arrows). (C) Quantification of the number of regenerated axons in vehicle and inhibitor treated mice. Vehicle n = 4, inhibitor n = 5. (D, E) Representative images of sectioned gastrocnemius muscles from vehicle (D) and inhibitor (E) treated mice stained for SV2, NF (both green), and α-BTX (red). (F) Quantification of the number of innervated NMJs between vehicle and inhibitor treated mice. Scale bars, 2 μm (A and B) and 50 μm (D and E). Vehicle n = 4, inhibitor n = 3. Numbers in parenthesis indicate total number of NMJs counted. Bars represent the mean ± standard error. Statistical significance was calculated using a two-tailed Student’s t test. *p < 0.05