Figure 4.

Blocking IFN-I signaling alters the amount of GzymB produced, but has little effect on the proportion of NK cells producing GzymB. PBMCs (n=12) were cultured in vitro with B18R (100 ng/ml) for 1 h, prior to stimulation with RV16 (MOI = 1), alongside an unstimulated control (US) for 24 h. (A) Percentage of GzymB-producing CD56⁺ (left), CD56^dim (middle), and CD56^bright (right) NK cells. (B) Amount (indicated by MFI) of intracellular GzymB in CD56⁺ (left), CD56^dim (middle), and CD56^bright (right) NK cells. Each colored symbol represents data from one donor, lines represent medians. Data are representative of three experiments. *p<0.05, **p<0.01, ***p<0.001 by Wilcoxon matched-pairs signed rank tests. IFN-I, type I interferon; GzymB, granzyme B; NK, natural killer; PBMC, peripheral blood mononuclear cell; UT, untreated; RV16, rhinovirus 16; MOI, multiplicity of infection; US, unstimulated; MFI, median fluorescence intensity.