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. 2020 Aug 25;103(5):966–977. doi: 10.1093/biolre/ioaa146

Figure 6.

Figure 6

Overexpression of FOXL2 impairs theca cell differentiation. (A) Immunofluorescence for the steroidogenic enzyme HSD3B (red), granulosa cell marker AMH (cyan) and germ cell marker GCNA1 (yellow) in control and FOXL2+ ovaries at postnatal day 9 (P9). Right panels are higher magnification of outlined areas. Arrows indicate HSD3B+ theca/interstitial cells. *: AMH-negative granulosa cells. Scale bar: 100 μm; n = 4/genotype. (B) Immunofluorescence for the steroidogenic enzyme CYP17A1 (red) and SMA (green) in control and FOXL2+ ovaries at P9. Right panels are higher magnification of outlined areas. Arrows indicate CYP17A1+ theca cells. Scale bar: 100 μm; n = 4/genotype. The yellow staining represents autofluorescent blood cells. (C-D) qPCR analysis of expression of steroidogenic enzymes Hsd3b, Cyp17a1, Cyp11a1 and Star (C) and granulosa cell genes Amh, Dhh and Ihh (D) in control and FOXL2+ ovaries at P9. The data were analyzed with a Student t-test; Bar graphs represent mean ± SEM (n = 7–8/genotype); ***P < 0.001; *P < 0.05.