FIGURE 5.

Offline analysis of growth and substrate consumption (OD₆₂₅, glucose and NaNO₃) for all seven Ustilaginaceae species in medium 1 (A) and medium 2 (B) with initial substrate concentrations of 30 g L^–1 glucose and 3 g L^–1 NaNO₃. Both substrates were efficiently consumed in medium 2 within 48 h. In medium 1, glucose and nitrate were still unconsumed after 48 h, most probably due to other organic nitrogen sources present in the yeast extract, which are consumed first.