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. 2020 Oct 21;8:555280. doi: 10.3389/fbioe.2020.555280

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Copyright © 2020 Beck and Zibek.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Composition of organic extracts from M. aphidis (A) U. siamensis (B), and P. hubeiensis pro tem. (C) cultures in different concentrations of medium 1 (upper) and medium 2 (lower), after initiation of MEL production with 8% v/v rapeseed oil as substrate. Relative amounts of unconsumed triglycerides (TAG), free fatty acids (FFAs) and the different MEL variants are shown. For M. aphidis and P. hubeiensis pro tem., a generally higher conversion from oil to MEL in the extracts was achieved with medium 2 compared to medium 1. Increasing medium concentration in growth phase, which led to higher biomass concentrations (see Figure 6), showed a positive effect on overall conversion of oil into MEL for all species, while the relative proportions of the MEL-variants MEL-A, -B, -C, -D remained constant.