Figure 10.

Effects of 25 mmol/L glucose and addition of 1 mmol/L nicotinamide or 25 µmol/L resveratrol on premature senescence in culture human retinal pericytes. Human retinal pericytes were cultured in 5% fetal bovine serum with EGM2 media for 10 days with the addition of indicated concentration of reagents. The cells were fixed and senescence associated galactosidase staining was carried out. Then, 25 mmol/L glucose increased a number of senescence cells by threefold (*P < 0.05 vs 5 mmol/L glucose, n = 4). The addition of 1 mmol/L nicotinamide in the medium further increased (*P < 0.05 vs 5 mmol/L glucose and ^† P < 0.05 vs 25 mmol/L glucose, n = 4); 20 µmol/L resveratrol treatment eliminated 25 mmol/L glucose effects (^† P < 0.05 vs 25 mmol/L glucose).