Table 1.

CTNNB1 exon 3 sequencing analysis of DNA from 22 cases of ACP

Case No:	Age at diagnosis (years)*	Tumour content (% nuclear area)	DNA conc (copies/ul)	CTNNB1 mutation by Tam‐Seq	CTNNB1 mutation by Sanger Seq	Average mutation allele frequency
1	8	20	1100	T41I	T41I	17%
2	14	70	2315	T41I	T41I	28%
3	6	70	390	S37F	No mutation	15%
4	14	60	1155	S45F	Failed**	35%
5	8	10	525	S33F	No mutation	3%
6	12	50	680	S37A	No mutation	16%
7	46	70	770	T41I	No mutation	21%
8	71	80	8854	T41I	T41I	35%
9	adult	80	395	S33C	S33C	39%
10	adult	80	2273	T41I	T41I	29%
11	60	70	143	T41I	T41I	15%
12	83	45	2087	S33C	S33C	19%
13	22	80	439	T41I	T41I	26%
14	87	90	1573	D32N	D32N	31%
15	61	50	195	S33C	S33C	30%
16	65	40	237	T41A	T41A	25%
17	53	40	520	S37A	S37A	17%
18	adult	90	470	I35S	Failed**	43%
19	adult	60	260	S33F	No mutation	25%
20	adult	25	1256	S37F	No mutation	3%
21	adult	20	1460	S33F	No mutation	18%
22	adult	70	4505	S37C	Failed**	47%

^*Age not available in all patients.

^**Sanger sequencing reaction failed to give readable trace.