Skip to main content
. 2020 Nov 3;40(11):BSR20200552. doi: 10.1042/BSR20200552

Figure 7. No synergy between C1 and C2 in TnT proteolysis.

Figure 7

(A) Proteolysis of TnT by C1 and/or C2. One microgram of TnT was incubated with C1 (6 mU), C2 (1.5 mU), or both for 20 min, followed by SDS-PAGE analysis. Reactions were performed by triplicate. Average intensities of the full-length (intact) and representative proteolyzed products of TnT (PP1 to 4) were quantitated. (B–F) Inhibitory effect of C2 on TnT proteolysis by C1. Proteolysis of TnT protein by C1 was suppressed in the presence of C2, as measured by the decrease in the level of intact TnT (B), as well as the amount of proteolyzed product (C–F). At the amount used, proteolysis of TnT by C2 was barely detectable (A, three lanes from the right); *, P<0.05; **, P<0.01.