Table 1. Summary of LC-MS analysis of TnT proteolysis by C1 or C2.
| Qualification | No. |
|---|---|
| Spectra | 39165 |
| Identified spectra | 9255 |
| peptides with FDR < 1% | 5082 |
| Distinct peptides | 1699 |
| Quantified distinct peptides with FDR < 1% * | 436 |
| TnT peptides clear the qualifications above | 242 |
| C1 peptides clear the qualifications above | 40 |
| C2 peptides clear the qualifications above | 38 |
*: Distinct peptides derived from identified proteins with acceptable modifications. Accepted modification: iTRAQ at N-terminus and/or Lys, oxidation at Met, methylthiolation at Cys, conversion of N-terminal Glu to PyroGlu.