Fig. 1.

iPEM assembled on gold nanoparticle templates promoted antigen-specific T Cell response. (A) The anionic adjuvant and cationic antigen peptides were self-assembled on gold nanoparticle templates via electrostatic interactions. (B) Confocal microscopy images demonstrated high levels of peptide and poly I:C located within DCs following a 3 h incubation with iPEM-GNPs. White: cell membrane; blue: nucleus; Green: peptide; Red: polyI:C; scale bars are 10 µm. (C) Mice were immunized with simple mixtures of soluble antigen and adjuvant or iPEM-GNPs on day 0 then boosted on day 14. Frequency of CD8⁺ T cells in peripheral blood over 28 days demonstrated immunization with iPEM-GNPs promoted efficient primary and secondary CD8⁺ T cell responses in mice. Reproduced with permission from [120]. Copyright 2015 American Chemical Society.