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Transient absorption spectra of as-prepared R. rubrum FixAB (red traces) and A. vinelandii FixABCX (black traces). (A) Kinetic traces of ASQ signal (dots) at 365 nm. ASQ decay shows half-lives of ~15 ps and ~1000 ps when fit with a double exponential function (solid lines). (B) An NSQ absorption peak (~565–650 nm) is only observed in the A. vinelandii FixABCX complex.
