Table 1. Data Collection and Refinement Statistics.
| Parameter | DNA-bound HD1CCT/GHD8/OsNF-YC2 | GHD8/OsNF-YC2 |
|---|---|---|
| Space group | P 31 2 1 | P 61 |
| Cell dimensions | ||
| a, b, c (Å) | 136.08, 136.08, 62.96 | 89.27, 89.27, 81.34 |
| α, β, γ (°) | 90, 90, 120 | 90, 90, 120 |
| Wavelength (Å) | 0.97914 | 0.97946 |
| Resolution (Å) | 45 ∼2.55 (2.66∼2.55) | 45∼2.00 (2.05∼2.00) |
| Rmerge (%) | 9.0 (80.0) | 8.2 (58.0) |
| Rpim (%) | 2.9 (26.7) | 2.6 (18.1) |
| I/σ | 14.8 (2.4) | 21.2(5.3) |
| Completeness (%) | 99.5 (99.2) | 99.7 (98.0) |
| Number of measured reflections | 217, 423 (24,970) | 270, 613 (19,817) |
| Number of unique reflections | 22, 041 (2,658) | 25, 020 (1,825) |
| Redundancy | 9.9 (9.4) | 10.8 (10.9) |
| Wilson B factor (Å2) | 45.5 | 22.3 |
| Rwork/Rfree (%) | 19.95/22.58 | 17.06/21.46 |
| Number of atoms | ||
| Protein | 1,708 | 2,737 |
| Main chain | 840 | 1,356 |
| Side chain | 868 | 1,381 |
| Water molecules | 16 | 168 |
| Other entities (DNA) | 1,025 | 0 |
| All atoms | 2,749 | 2,905 |
| Average B value (Å2) | ||
| Protein | 67.9 | 36.7 |
| Main chain | 64.6 | 31.9 |
| Side chain | 71.1 | 41.5 |
| Water molecules | 63.0 | 46.3 |
| Other entities (DNA) | 77.7 | 0 |
| All atoms | 71.5 | 37.3 |
| Root mean square deviations | ||
| Bonds (Å) | 0.010 | 0.007 |
| Angle (°) | 1.094 | 0.853 |
| Ramachandran plot statistics (%) | ||
| Most favorable | 96.1 | 98.5 |
| Additionally allowed | 3.9 | 1.5 |
| Generously allowed | 0 | 0 |
Values in parentheses are for the highest resolution shell. Rmerge = ΣhΣi|Ih,i-Ih|/ΣhΣiIh,i, where Ih is the mean intensity of the i observations of symmetry-related reflections of h. R = Σ|Fobs − Fcalc|/ΣFobs, where Fcalc is the calculated protein structure factor from the atomic model (Rfree was calculated with 5% of the reflections selected).