Figure 2.

OsHKT1;5 Acts Genetically Downstream of OsBAG4.

(A) Histograms of fold changes for up- and downregulated DEGs in shoot and root comparing osbag4-1 and NIP. “x axis” shows the bins of log₂(Fold change) in RNA expression, and “y axis” is the number of DEGs whose log₂(Fold change) expression fall in each bin.

(B) Expression levels of OsHKT family genes in the root or shoot of NIP and osbag4-1. Data represent means ± sd (n = 3, roots or shoots from five NIP or osbag4-1 seedlings were pooled and harvested for RNA extraction and RT-qPCR in each biological replicate). Statistical analyses were performed using Student’s t test. Individual values (black circle) are shown.

(C) and (D) Box plot showing K⁺ (C) and Na⁺ (D) contents in the shoots of the indicated genotypes. Four-week-old seedlings were treated with or without 100 mM NaCl for 5 d before measurement of ion contents (n = 8, eight plants of each genotype were used to measure Na⁺ and K⁺ levels). Differences between NIP and mutants or complementation lines were evaluated with Student’s t test. DW, dry weight.

(E) and (F) Images (E) and survival rates (F) of NIP, osbag4-1, oshkt1;5-1, and osbag4-1 oshkt1;5-3 double mutant before 100 mM NaCl treatment and after recovery from NaCl treatment. Data in (F) represent means ± sd (n = 5, five biological experiments were performed with 24 plants in each). Individual values (black circle) are shown. Statistical analyses were performed by Student’s t test. Bars in (E) = 3.2 cm.