Figure 3.

PCA of ¹³C-Labeled Metabolites.

(A) to (F) S. bicolor (see [A] and [B]) and T. triandra (see [C] to [F]). Values for awn and SS are not significantly different in either species for any time point nor for most individual metabolites. (A), (C), and (E) Average labeling. Each point is the weighted average of all labeled isotopologues for a given metabolite, organ, and time point. (B), (D), and (F) All isotopologues.

(A) Values for PS are significantly different from the other organs. See Supplemental Table 4.

(B) Awn and SS are significantly different for P5P and UDPG, but not otherwise; PS is significantly different from the other two organs except for PYR. Values at 30 and 60 s not significantly different for most metabolites, but the 300-s time point is distinct. See Supplemental Table 5.

(C) Values for PS are significantly different for six of nine metabolites, with the greatest difference in labeling at 300 s. See Supplemental Table 6.

(D) Awn and SS are significantly different for P5P, but not otherwise; PS is significantly different from the other two organs except for P5P, PGA, and PYR. Values at 30 and 60 s are similar for most metabolites, but the 300-s time point is distinct. See Supplemental Table 7.

(E) and (F) Values including bract; data for awn, SS and PS are the same as those in (C). (E) Bract is significantly different from all other organs for six out of eight metabolites. Values for PS are significantly different from awn and SS for only aspartate and PYR. The 300-s time point is significantly different from others. See Supplemental Table 11.

(F) Results are similar to those for average labeling, with bract being significantly different from all other organs for six out of eight metabolites and isotoplogues, and the greatest difference in labeling occurring at 300 s. See Supplemental Table 12.

Organs are distinguished by color, and time points are distinguished by shape. 30, values at 30 s of labeling; 60, values at 60 s of labeling; 300, values at 300 s of labeling.