Figure 6.

Immunofluorescence of Rubisco Large Subunit in Sorghum Spikelets.

(A), (C), (E), (G), and (I) Cross section of glume of PS.

(B), (D), (F), (H), and (J) Cross section of glume of SS.

(A) and (B) Bright field.

(C) and (D) Anti-Rubisco large subunit with Alexa 488 as the secondary antibody; emission window 509 to 585 nm.

(E) and (F) Lignin autofluorescence; emission window 415 to 485 nm.

(G) and (H) Chlorophyll autofluorescence; emission window 661 to 779 nm. Autofluorescence in sclerenchyma in (H) is nonspecific.

(I) and (J) Merge of Rubisco, chlorophyll, and lignin channels. Black arrowheads, vascular bundles; inner (adax), facing the inside of the spikelet; outer (abax), facing the outside of the spikelet; bs and white arrows, bundle sheath; scl, sclerenchyma. Bars = 50 µm.