Figure 3.

Complementation of Light Stress–Induced SAA in the rbohD Mutant with RBOHD Driven by Different Tissue-Specific Promoters.

Light stress–induced systemic leaf cell injury (measured as electrolyte leakage) of the wild type (Columbia-0 [Col]), rbohD, and the different rbohD complemented Arabidopsis plants was determined. Systemic leaves were either untreated, nonpretreated, and unstressed (untreated, unstressed) or subjected to light stress following a pretreatment of one local leaf with light stress (pretreated, stressed). SAA is evident by a low level of electrolyte leakage in light-stressed systemic leaves (pretreated, stressed), which is similar to that of untreated and unstressed leaves (untreated, unstressed). Ten different plants each from two independent complemented lines for each construct (nos. 1 and 2) were subjected to light stress, and cell injury was measured in systemic leaves by electrolyte leakage. Student’s t test, sd, n = 20, *P < 0.05. CAB, CHLOROPHYLL A/B BINDING PROTEIN; CER, ECERIFERUM; SCR, SCARECROW; SULTR, SULFATE TRANSPORTER; XCP, XYLEM CYSTEINE PEPTIDASE.