Figure 4.

RBOHD and RBOHF Are Required for Mediating Systemic ROS Signaling in Response to a Local HL Stress Treatment.

Representative time-lapse images of whole-plant ROS levels (indicated by DCF oxidation) of the wild type (Columbia-0 [Col]), rbohD, rbohF, and rbohD robhF double mutant Arabidopsis plants subjected to a 2-min local HL stress treatment (applied to the local leaf only) are shown on left; representative line graphs showing continuous measurements of ROS levels in local and systemic leaves of the wild type, rbohD, rbohF, and rbohD robhF mutants, over the entire course of the experiment (0 to 30 min), are shown in the middle (ROIs for some of them are indicated with blue boxes); and statistical analysis of ROS levels in local and systemic leaves at 0 and 30 min is shown on right (conducted for 10 different plants from the wild type and each of the different mutants; Student’s t test, sd, n = 10, *P < 0.05). All experiments were repeated at least three times with similar results. Treated local leaves are indicated with a red circle. RBOHF, RESPIRATORY BURST OXIDASE HOMOLOG F; ROI, region of interest; TRE, total radiant efficiency.