Figure 5.

Detection of ROS in Petioles from Local and Systemic Leaves of the Wild-Type, rbohD, rbohF, and rbohD rbohF Double Mutant Plants Subjected to a 2-Min HL Stress Treatment (Applied to the Local Leaf Only).

(A) Representative fluorescence microscopy images of ROS detection in petioles of the wild type and the different mutants subjected to a 2-min local HL stress treatment. Bar = 1000 µm.

(B) Bar graph of fold change in fluorescent intensity in veins and epidermis of the wild type (Columbia-0 [Col]) and the different mutants subjected to a 2-min HL stress treatment (applied to the local leaf only; conducted for 10 different plants each from the wild type and the different rboh mutants; Student’s t test, sd, n = 10, *P < 0.05). RBOHF, RESPIRATORY BURST OXIDASE HOMOLOG F.