qua2 and tsd2 Mutants Have Shorter Hypocotyls and Show Cell Adhesion Defects.
(A) Six-day-old etiolated Arabidopsis Col, qua2, and tsd2 seedlings. Bar = 1 cm.
(B) Quantification of hypocotyl length in dark-grown seedlings 2 to 6 d after sowing (DAS; n ≥ 30 seedlings per genotype per time point). qua2 and tsd2 hypocotyls were much shorter than Col controls, and tsd2 hypocotyls were shorter than qua2 hypocotyls. Error bars represent sd. *, P < 0.05 and **, P < 0.001, Student’s t test.
(C) Hypocotyls from 3-d-old dark-grown Col, qua2, and tsd2 seedlings. Bar = 100 µm.
(D) Images of stomata present on the abaxial side of cotyledons of 6-d-old light-grown Col, qua2, and tsd2 seedlings. Cells were labeled with 100 µg/mL propidium iodide and imaged by spinning-disk confocal microscopy. Bar = 10 µm.