Figure 2.

NIGT1.2 Positively Modulates Pi Acquisition in Arabidopsis during Pi Starvation.

(A) GUS staining assay of the ProNIGT1.2:GUS transgenic line. Seven-day-old ProNIGT1.2:GUS seedlings were transferred to MS or low-Pi (LP) medium for 5 d and then stained for GUS.

(B) Analysis of NIGT1.2 expression using RT-qPCR in 10-d-old NIGT1.2-overexpressing and wild-type plants. Data are shown as mean ± se (n = 3).

(C) Measurement of Pi concentration. Seven-day-old seedlings were transferred to MS medium for 5 d and then harvested for Pi concentration measurement. Data are shown as mean ± se (n = 3).

(D) ³²Pi uptake capacity was measured in 10-d-old seedlings germinated and grown on MS medium. Data are shown as mean ± se (n = 3).

(E) Phenotypic comparison of the nigt1.2, nigt1.1, nigt1.1 nigt1.2 double mutants, and wild-type seedlings during Pi starvation. Seven-day-old seedlings were transferred to LP or MS medium for another 7 d, and then photographs were taken.

(F) Pi concentration measurement in 7-d-old seedlings grown on MS or LP medium for 5 d. Data are shown as mean ± se (n = 4).

(G) ³²Pi uptake capacity was measured in 7-d-old seedlings grown on MS or LP medium for 3 d. Data are shown as mean ± se (n = 4). Asterisks in (C), (D), (F), and (G) indicate significant differences compared with wild-type plants (^#) by Student’s t test: *P < 0.05; **P < 0.01.