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. 2020 Sep 24;7(21):2002524. doi: 10.1002/advs.202002524

Figure 1.

Figure 1

Overview of the multimodal and multiscale approach. A) Scheme of the hierarchical structural organization in embryonic long bone. At the macro‐scale, the rudiment is composed of a cartilage template and a mineralized region originating from the middle of the rudiment (primary bone). At the mesoscale, bone can be divided into two subgroups; trabecular and cortical‐like bone (bone collar). Both subgroups are composed of mineralized collagen fibrils at the microscale. The nanoscaled mineral platelets embedded in the collagen fibril network is mainly constituted by Ca (forming HA: Ca5(PO4)3(OH)). B) Overview of the techniques used in this study to probe the structure and composition of the different length scales. The overall morphology and inner structure were obtained using μCT with 0.65, 0.81 and 1.625 µm spatial resolution. The molecular composition was probed by FTIR with 4.6 µm spatial resolution. The mineral platelet dimensions were determined with a 2 µm spatial resolution using SAXS/WAXS. Elemental composition was evaluated using XRF, at both 2 µm and 200 nm spatial resolutions.